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EN 1276:2019
EN 1276:2019
Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas.
II Phase / I step
Test organisms:
Enterococcus hirae ATCC 10541
Pseudomonas aeruginosa ATCC 15442
Staphylococcus aureus ATCC 6538
Escherichia coli K12 ATCC 10538

Temperature (°C): 20
Contact time (min.): 5
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l

Reduction (lg): ≥ 5,0
The following additional test conditions are permitted:
Salmonella typhimurium ATCC 13311
Lactobacillus brevis DSM 6235
Enterobacter cloacae DSM 6234
Other additional strains are permitted

Temperature (°C): 4, or 10, or 40
Contact time (min.): 1, or 15, or 30, or 60
*or client requirements

Interfering substances:
1% milk or 10 g/l yeast extract or 10 g/l sucrose or pH 5 and pH 9 buffer solutions or 5 g/l laurylsulfate
Reduction (lg): ≥ 5,0

Chemical disinfectants and antiseptics Phase I tests

Quantitative suspension tests to establish that a product has biocidal activity without regard to specific conditions of intended use.

Chemical disinfectants and antiseptics Phase II / step 1 tests

Quantitative suspension tests to establish that a product has biocidal activity simulating practical conditions appropriate to its intended use.

Chemical disinfectants and antiseptics Phase II / step 2 tests

Quantitative laboratory tests simulating practical conditions e.g. surface, instruments, handwash and handrub tests.

Chemical disinfectants and antiseptics other tests

Other chemical disinfectants and antiseptics tests.