A.
Test organisms:
Staphylococcus aureus ATCC 6538
Pseudomonas aeruginosa ATCC 15442
Escherichia coli K12 ATCC 10538
Enterococcus hirae ATCC 10541
Temperature (°C): 20
Contact time (min.): 5
*or client requirements
Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l
Reduction (lg): ≥ 4,0
The following additional test conditions are permitted:
Salmonella typhimurium ATCC 13311
Lactobacillus brevis DSM 6235
Enterobacter cloacae DSM 6234
Other additional strains are permitted
Temperature (°C): 4, or 10, or 40
Contact time (min.): 1, or 15, or 30, or 60
*or client requirements
Interfering substances:
1% milk or 10 g/l yeast extract or 10 g/l sucrose or pH 5 and pH 9 buffer solutions or 5 g/l laurylsulfate
Reduction (lg): ≥ 4,0
Staphylococcus aureus ATCC 6538
Pseudomonas aeruginosa ATCC 15442
Escherichia coli K12 ATCC 10538
Enterococcus hirae ATCC 10541
Temperature (°C): 20
Contact time (min.): 5
*or client requirements
Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l
Reduction (lg): ≥ 4,0
The following additional test conditions are permitted:
Salmonella typhimurium ATCC 13311
Lactobacillus brevis DSM 6235
Enterobacter cloacae DSM 6234
Other additional strains are permitted
Temperature (°C): 4, or 10, or 40
Contact time (min.): 1, or 15, or 30, or 60
*or client requirements
Interfering substances:
1% milk or 10 g/l yeast extract or 10 g/l sucrose or pH 5 and pH 9 buffer solutions or 5 g/l laurylsulfate
Reduction (lg): ≥ 4,0
B.
Test organisms:
Aspergillus brasiliensis (previously A. niger) ATCC 16404
Candida albicans ATCC 10231
Temperature (°C): 20
Contact time (min.): 15
*or client requirements
Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l
Reduction (lg): ≥ 3,0
The following additional test conditions are permitted:
Saccharomyces cerevisiae ATCC 9763
Saccharomyces cerevisiae DSM 70487
var. diastaticus
Additional strains are permitted
Temperature (°C): 4, 10, 40
Contact time (min.): 1, 15, 30, 60
*or client requirements
Interfering substances:
1% milk or 10 g/l yeast extract or 10 g/l sucrose or pH 5 and pH 9 buffer solutions or 5 g/l laurylsulfate
Reduction (lg): ≥ 3,0
Aspergillus brasiliensis (previously A. niger) ATCC 16404
Candida albicans ATCC 10231
Temperature (°C): 20
Contact time (min.): 15
*or client requirements
Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l
Reduction (lg): ≥ 3,0
The following additional test conditions are permitted:
Saccharomyces cerevisiae ATCC 9763
Saccharomyces cerevisiae DSM 70487
var. diastaticus
Additional strains are permitted
Temperature (°C): 4, 10, 40
Contact time (min.): 1, 15, 30, 60
*or client requirements
Interfering substances:
1% milk or 10 g/l yeast extract or 10 g/l sucrose or pH 5 and pH 9 buffer solutions or 5 g/l laurylsulfate
Reduction (lg): ≥ 3,0