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EN 14476:2013+A2:2019
EN 14476:2013+A2:2019
Quantitative suspension test for the evaluation of virucidal activity in the medical area
II Phase / I step
Hygienic handrub and handwash
Test organism:
Poliovirus
Adenovirus
Murine Norovirus
*Limited spectrum virucidal activity - Adenovirus
*Activity against enveloped viruses - Vacciniavirus

Temperature (°C):
20
Contact time (min.):
According to the manufacturer`s recommendation, but between 30 s and 120 s
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l + erythrocytes 3,0 ml/l

Reduction (lg): ≥ 4,0
Textile disinfection
Test organisms: Parvovirus
Temperature (°C):
According to the manufacturer`s recommendation, but between 30 and 70
Contact time (min.):
According to the manufacturer`s recommendation, but no longer than 20
*or client requirements

Interfering substances:
Dirty conditions – bovine albumin 3,0 g/l + erythrocytes 3,0 ml/l
Reduction (lg): ≥ 4,0
Instrument disinfection
Test organisms:
Poliovirus
Adenovirus
Murine Norovirus
when temperature is 40 °C or higher - only Parvovirus

Temperature (°C):
According to the manufacturer`s recommendation, but between 20 and 70
Contact time (min.):
According to the manufacturer`s recommendation, but no longer than 60
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l + erythrocytes 3,0 ml/l

Reduction (lg): ≥ 4,0
Surface disinfection
Test organisms:
Poliovirus
Adenovirus
Murine Norovirus
*Limited spectrum virucidal activity - Adenovirus
* Activity against enveloped viruses - Vacciniavirus

Temperature (°C):
According to the manufacturer`s recommendation, but between 4 and 30
Contact time (min.):
According to the manufacturer`s recommendation, but no longer than 5 or 60
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l + erythrocytes 3,0 ml/l

Reduction (lg): ≥ 4,0

Chemical disinfectants and antiseptics Phase I tests

Quantitative suspension tests to establish that a product has biocidal activity without regard to specific conditions of intended use.

Chemical disinfectants and antiseptics Phase II / step 1 tests

Quantitative suspension tests to establish that a product has biocidal activity simulating practical conditions appropriate to its intended use.

Chemical disinfectants and antiseptics Phase II / step 2 tests

Quantitative laboratory tests simulating practical conditions e.g. surface, instruments, handwash and handrub tests.

Chemical disinfectants and antiseptics other tests

Other chemical disinfectants and antiseptics tests.