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EN 13704:2018
EN 13704:2018
Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas
II Phase / I step
Test organisms:
Bacillus subtilis ATCC 6633
Temperature (°C): 20
Contact time (min.): 60
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Reduction (lg): ≥ 3,0
The following additional test conditions are permitted:
Bacillus cereus ATCC 12826
Clostridium sporogenes 51 CIP 7939

Temperature (°C): 4, or 10, or 40, or 75
Contact time (min.): 1, or 15, or 30
*or client requirements

Reduction (lg): ≥ 3,0

Chemical disinfectants and antiseptics Phase I tests

Quantitative suspension tests to establish that a product has biocidal activity without regard to specific conditions of intended use.

Chemical disinfectants and antiseptics Phase II / step 1 tests

Quantitative suspension tests to establish that a product has biocidal activity simulating practical conditions appropriate to its intended use.

Chemical disinfectants and antiseptics Phase II / step 2 tests

Quantitative laboratory tests simulating practical conditions e.g. surface, instruments, handwash and handrub tests.

Chemical disinfectants and antiseptics other tests

Other chemical disinfectants and antiseptics tests.