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EN 14349:2012
EN 14349:2012
Quantitative surface test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary field on non-porous surfaces without mechanical action
II Phase / II step
Test organisms:
Enterococcus hirae ATCC 10541
Proteus vulgaris ATCC 13315
Pseudomonas aeruginosa ATCC 15442
Staphylococcus aureus ATCC 6538

Temperature (°C): 10
Contact time (min.): 30
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 3 g/l
Dirty conditions – 10 g/l bovine albumin and 10 g/l yeast extract

Reduction (lg): ≥ 4,0
The following additional test conditions are permitted:
Additional test organisms
Temperature (°C): 4, 20, 40
Contact time (min.): 1, 5, 60
*or client requirements

Chemical disinfectants and antiseptics Phase I tests

Quantitative suspension tests to establish that a product has biocidal activity without regard to specific conditions of intended use.

Chemical disinfectants and antiseptics Phase II / step 1 tests

Quantitative suspension tests to establish that a product has biocidal activity simulating practical conditions appropriate to its intended use.

Chemical disinfectants and antiseptics Phase II / step 2 tests

Quantitative laboratory tests simulating practical conditions e.g. surface, instruments, handwash and handrub tests.

Chemical disinfectants and antiseptics other tests

Other chemical disinfectants and antiseptics tests.