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EN 16437:2014+A1:2019
EN 16437:2014+A1:2019
Quantitative surface test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in veterinary area on porous surfaces without mechanical actionareas
II Phase / II step
Test organisms:
Enterococcus hirae
Proteus vulgaris
Pseudomonas aeruginosa
Staphylococcus aureus
Temperature (°C): 10
Contact time (min.): 1
*or client requirements

Interfering substances:
Bovine albumin 3,0 g/l
Reduction (lg): ≥ 4,0
The following additional test conditions are permitted:
Any relevant test organism
Temperature (°C): 4, or 20, or 40
Contact time (min.): 5, or 15, or 30, or 60, or up to 330 min at 30 min intervals, max. 360 min
*or client requirements

Interfering substances:
Any relevant substance
Reduction (lg): ≥ 4,0

Chemical disinfectants and antiseptics Phase I tests

Quantitative suspension tests to establish that a product has biocidal activity without regard to specific conditions of intended use.

Chemical disinfectants and antiseptics Phase II / step 1 tests

Quantitative suspension tests to establish that a product has biocidal activity simulating practical conditions appropriate to its intended use.

Chemical disinfectants and antiseptics Phase II / step 2 tests

Quantitative laboratory tests simulating practical conditions e.g. surface, instruments, handwash and handrub tests.

Chemical disinfectants and antiseptics other tests

Other chemical disinfectants and antiseptics tests.