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EN 16438:2014
EN 16438:2014
Quantitative surface test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the veterinary area on non-porous surfaces without mechanical action
II Phase / II step
Test organisms:
Aspergilus brasiliensis (conidiospores)
Candida albicans (vegetative cells)

Temperature (°C): 10
Contact time (min.): 60
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 3,0 g/l
Dirty conditions – 10 g/l yeast extract plus bovine albumin 10 g/l

Reduction (lg): ≥ 3,0
The following additional test conditions are permitted:
Test organisms:
Any relevant test organism
Temperature (°C): 4, or 20, or 40
Contact time (min.): 5, or 30, or 120
*or client requirements

Interfering substances:
Any relevant substance
Reduction (lg): ≥ 3,0

Chemical disinfectants and antiseptics Phase I tests

Quantitative suspension tests to establish that a product has biocidal activity without regard to specific conditions of intended use.

Chemical disinfectants and antiseptics Phase II / step 1 tests

Quantitative suspension tests to establish that a product has biocidal activity simulating practical conditions appropriate to its intended use.

Chemical disinfectants and antiseptics Phase II / step 2 tests

Quantitative laboratory tests simulating practical conditions e.g. surface, instruments, handwash and handrub tests.

Chemical disinfectants and antiseptics other tests

Other chemical disinfectants and antiseptics tests.